[最新] oil red o staining protocol isopropanol 332511-Oil red o staining protocol isopropanol

1 Saturated stock solution of oil redO (025 05%) in isopropyl alcohol Dilute 6 ml of stock solution oil redO with 4 ml dH, let stand for 5 10 min Filter the diluted oil redO (solution Add 2 ml of 60% Isopropanol to cover the bottom of each well and let sit for 25 minutes Pour off the Isopropanol and pipet 2 ml of the working solution of oil red o along theOil Red O Stock Solution – Reconstitute with mL of 100% isopropanol Mix well and leave undisturbed for minutes to make the Oil Red O Stock Solution The Oil Red O Stock Solution

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Oil red o staining protocol isopropanol

Oil red o staining protocol isopropanol- Prepare oil red O 05g in 100% isopropanol Fix cells using 4% paraformaldehyde (4 degrees Celsius) or 10% phosphate buffered formalin (room temp) After fixing for 1530 min1 Prepare fresh Working Oil Red O Isopropanol Solution;

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Allow to stand at room temperatureOil red O solution has been used to stain neutral lipids and adipocytes and to visualize fat droplets in fibroblasts Oil red O is a lysochrome (fatsoluble dye) diazo dye used for staining of neutralElute Oil Red O dye by adding 1 ml of 100% isopropanol and incubate for 10 min with gently shaking Pipet the isopropanol with Oil Red O up and down several times to ensure that all Oil Red O is in

3 A few dips in 60% isopropyl alcohol 4 Oil red O working solution for minutes 5 A few dips in 60% isopropyl alcohol 6 Rinse with four changes of distilled water 7 Counterstain with 1 Rinse slides in distilled water 2 Rinse with 60% isopropyl alcohol to clear background 3 Stain with freshly prepared Oil Red O working solution 15 mins 4 Rinse briefly5 min Remove isopropanol and add Oil Red O Working Solution to completely and evenly cover the cells Rotate plate or dish and incubate for 10 min Remove Oil Red O solution and wash 25X

Preheat Oil Red O Stain to 60°C Place frozen sections in 10% Formalin for 25 minutes then rinse slide in tap water (fixed slide may skip this step) Circle the tissue by Dako Pen Place slide inIcp is associated factors affect millions of oil red o quantification protocol isopropanol was estimated by the authors Online Gaming Software Nbf is smaller lds may not add any liquid filmStain in filtered Working Oil Red O Isopropanol Solution (Step #1) for 10 to 15 minutes Rinse in fresh Alcohol Isopropyl, 60% (Step #2) Wash thoroughly in distilled water Counterstain with

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Oil redO (ORO) was dissolved in three different solvents isopropanol, propylene glycol and triethyl phosphate At 48 h, the proliferative cultures were stained with the three stains ORO stainDissolve the dye in the isopropanol, using the very gentle heat of a water bath This is the stock stain CARE fire hazard 2 Oil Red O working solution For use Dilute 30 ml of the stock stainCombine and mix well a Oil Red O Stain, Isopropanol 30 ml b Distilled Water ml c Cover solution;

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Oil Red O Stock Solution 300mg Oil Red O 100mL 99% isopropanol Staining Solution 6 parts of stock Oil Red O 4 parts of distilled water Allow to stand for 10min Filter this solution withElute Oil Red O by adding 100% isopropanol, incubate about 10 m in (can be longer) Pipet the isopropanol with Oil Red O up and down several times to be sure that all Oil Red O is in theO Saturated Oil Red O in 99% Isopropanol (300mg of Oil Red O in /100mL isopropanol) • Working Oil Red O solution o 30 mL of stock solution mixed with mL of Distilled Water o Mix and let

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